| Popis: |
We demonstrate a fiber-based quantitative phase imaging (QPI) system with epi-illumination to acquire tissue and cellar level structure. Our approach is based on quantitative oblique back-illumination microscopy (qOBM), which keeps the advantages of QPI—label-free and non-destructive with nanometer-scale sensitivity—while also delivering tomographic sectioning capabilities in thick scattering samples using epi-illumination. The developed system uses a simple and robust configuration consisting of a flexible fiber bundle and a GRIN lens. Here data are presented with histopathological feature analysis. This technique, with its compact setup and real-time processing algorithm, can lead to in-vivo medical diagnosis, for clinical surgery and endoscopy. |
