Nucleotide Sequence of the Gene Coding for Proteolytic (Group I) Clostridium botulinum Type F Neurotoxin: Genealogical Comparison with other Clostridial Neurotoxins

Autor: Roger A. Hutson, Nicola J. Bodsworth, Michael J. Elmore, Matthew D. Collins, Sarah M. Whelan, Nigel P. Minton
Rok vydání: 1995
Předmět:
Zdroj: Systematic and Applied Microbiology. 18:23-31
ISSN: 0723-2020
DOI: 10.1016/s0723-2020(11)80444-3
Popis: Summary Botulinum neurotoxin type F can be produced by proteolytic or non-proreolytic strains of C. botulinum , or from C. baratii . In recent years, the sequences of all toxin types except that of proteolytic type F toxin have been determined. We have cloned the neurotoxin gene from proteolytic C. botulinum (type F Langeland) as a series of overlapping PCR fragments, and determined the nucleotide sequence. The translated sequence demonstrates a toxin of 1278 amino acid residues, which has highest similarity with non-proteolytic type F toxin. Phylogenetic trees based on toxin sequences show that the type F sequences fall into a naturally distinct group, although the strains producing these toxins are not phylogenetically closely related.
Databáze: OpenAIRE