Autor: |
Roger A. Hutson, Nicola J. Bodsworth, Michael J. Elmore, Matthew D. Collins, Sarah M. Whelan, Nigel P. Minton |
Rok vydání: |
1995 |
Předmět: |
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Zdroj: |
Systematic and Applied Microbiology. 18:23-31 |
ISSN: |
0723-2020 |
DOI: |
10.1016/s0723-2020(11)80444-3 |
Popis: |
Summary Botulinum neurotoxin type F can be produced by proteolytic or non-proreolytic strains of C. botulinum , or from C. baratii . In recent years, the sequences of all toxin types except that of proteolytic type F toxin have been determined. We have cloned the neurotoxin gene from proteolytic C. botulinum (type F Langeland) as a series of overlapping PCR fragments, and determined the nucleotide sequence. The translated sequence demonstrates a toxin of 1278 amino acid residues, which has highest similarity with non-proteolytic type F toxin. Phylogenetic trees based on toxin sequences show that the type F sequences fall into a naturally distinct group, although the strains producing these toxins are not phylogenetically closely related. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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