Characteristics of Bone Marrow Progenitor Cells of C57BL/6N Mice after 30-Day Hindlimb Suspension and 12-Hour Readaptation to Support Loading

Autor: Ludmila Buravkova, O. Yu. Alekseeva, Irina V. Andrianova, Elena R. Andreeva, E. A. Markina, P. I. Bobyleva
Rok vydání: 2020
Předmět:
Zdroj: Cell and Tissue Biology. 14:91-101
ISSN: 1990-5203
1990-519X
DOI: 10.1134/s1990519x20020078
Popis: The functional status of bone marrow (BM) progenitor cells, which provide hematopoiesis and homeostasis of bone tissue, determines many processes which take a place in spaceflight (SF) and subsequent recovery to gravity. It is known that SF factors and simulation of their effects do irreversible damage to the pool of hematopoietic and stromal progenitor cells. Meanwhile, changes in the functional activity of BM progenitor cells after simulation of SF effects were more pronounced than in the case of a real SF. The aim of the study was to examine processes of recovery of the functional activity of BM progenitor cells during readaptaion to loading after 30-day hindlimb suspension. Studies were performed on three groups of male mice: vivarium control, suspension, and readaptation. The following characteristics were monitored: number of nucleated BM cells, immunophenotype, proliferative activity of stromal BM cells, number of hematopoietic colony-forming units (CFU), CFUs of fibroblasts, and differentiating capabilities of BM hematopoietic and stromal precursors. Hindlimb suspension resulted in decreased number of fibroblast and hematopoietic CFU, inhibition of spontaneous osteodifferentiation of stromal progenitors and decreased expression of stromal markers. The readaptaion period was accompanied with restoration of the functional activity of stromal progenitors, while complete recovery of hematopoiesis was not observed.
Databáze: OpenAIRE
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