The effect of artichoke (Cynara scolymusL.) extract on ROS generation in HUVEC cells
| Autor: | Marek Droździk, Bogusław Czerny, Andrzej Pawlik, Zygmunt Juzyszyn |
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| Rok vydání: | 2008 |
| Předmět: |
Pharmacology
chemistry.chemical_classification Reactive oxygen species Antioxidant Endothelium biology medicine.medical_treatment Cytochrome c Pharmacognosy Molecular biology In vitro chemistry.chemical_compound medicine.anatomical_structure chemistry Dichlorofluorescein Cell culture Botany medicine biology.protein |
| Zdroj: | Phytotherapy Research. 22:1159-1161 |
| ISSN: | 0951-418X |
| DOI: | 10.1002/ptr.2385 |
| Popis: | The effect of an artichoke extract on induced reactive oxygen species (ROS) generation in cultured human umbilical endothelial cells (HUVECs) and its reductive properties were evaluated. Preincubation of HUVEC cells with the artichoke extract at concentrations of 25-100 microg/mL for 24 h abolished ROS generation induced by LPS and oxyLDL as evaluated by the fluorescence intensity of 2',7'-dichlorofluorescein (DCF). Potent, concentration-dependent reductive properties of the artichoke extract were demonstrated by the reduction kinetics of cytochrome c in reference to ascorbate were also revealed. The results of the present study the warrant application of artichoke extracts as endothelium protecting agents. |
| Databáze: | OpenAIRE |
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