Colistin- and carbapenem-resistant Klebsiella oxytoca harboring blaVIM-2 and an insertion in the mgrB gene isolated from blood culture
| Autor: | Katharina Richert, Yvonne Pfeifer, Udo Reischl, Andreas Hiergeist, André Gessner, Holger Melzl, Linda Falgenhauer, Michaela Simon, Jonathan Jantsch, Kornelius Fuchs, Roman G. Gerlach |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Bacilli 030106 microbiology Microbiology Lipid A 03 medical and health sciences Plasmid polycyclic compounds medicine Blood culture Gene biology medicine.diagnostic_test Klebsiella oxytoca General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Virology Multiple drug resistance Infectious Diseases Colistin bacteria medicine.drug |
| Zdroj: | International Journal of Medical Microbiology. 307:113-115 |
| ISSN: | 1438-4221 |
| Popis: | A carbapenemase-producing colistin-resistant Klebsiella oxytoca isolate was recovered from a blood culture of a female patient without previous report of risk factors to obtain multidrug-resistant Gram-negative bacilli. A combination of biochemical and molecular methods was used to identify the resistance mechanism of this isolate. Carbapenemase production was mediated by Verona integron-encoded metallo-β-lactamase (VIM)-2. Colistin resistance was not due to plasmid- borne mcr-1 gene, but we found an integration of IS5-like sequence in the mgrB gene of K. oxytoca. This gene is known to be an important regulator of the PhoPQ two-component system, and the disruption of this gene is most likely the cause of lipid A modification resulting in colistin resistance of our isolate. To the best of our knowledge this constitutes the first report of a carbapenemase-producing K. oxytoca with colistin resistance, a case that demonstrates the limited treatment options for infections with multidrug-resistant organisms. |
| Databáze: | OpenAIRE |
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