Autor: Jong Cheol Kim, Moo Je Cho, Yong Hwa Cheong, Yun Hwan Kang, Cha Young Kim, Sang Yeol Lee, Yoon Duck Koo, Min Chul Kim, Ja Choon Koo, Woo Sik Chung, Chae Oh Lim, Jae Hyuk Yoo, Hyun Jin Chun, Hyeong Cheol Park, Sung Chul Koo
Rok vydání: 2002
Předmět:
Zdroj: Plant Molecular Biology. 50:57-68
ISSN: 0167-4412
DOI: 10.1023/a:1016005231852
Popis: We isolated a stamen-specific cDNA, BSD1 (Brassica stamen specific plant defensin 1) that encodes a novel plant defensin peptide in Chinese cabbage (Brassica campestris L. ssp. pekinensis). Plant defensins are antimicrobial peptides containing eight highly conserved cysteine residues linked by disulfide bridges. In BSD1, the eight cysteine residues and a glutamate residue at position 29 are conserved whereas other amino acid residues of the plant defensins consensus sequence are substituted. BSD1 transcripts accumulate specifically in the stamen of developing flowers and its level drops as the flowers mature. The recombinant BSD1 produced in Escherichia coli showed antifungal activity against several phytopathogenic fungi. Furthermore, constitutive over-expression of the BSD1 gene under the control of the cauliflower mosaic virus (CaMV) 35S promoter conferred enhanced tolerance against the Phytophthora parasitica in the transgenic tobacco plants.
Databáze: OpenAIRE
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