Biospecific assembling of gold nanoparticles with protein or oligonucleotide linkers as studied by light scattering and extinction spectra

Autor: Nikolai G. Khlebtsov, Vladimir K. Plotnikov, Lev A. Dykman, Vladimir A. Bogatyrev, Yaroslav M. Krasnov
Rok vydání: 2002
Předmět:
Zdroj: SPIE Proceedings.
ISSN: 0277-786X
Popis: In this work, we report on the optical properties of colloidal gold bioconjugates obtained by attaching protein molecules or oligonucleotide probes to the surface of the particles. Addition of complementary components to the bioconjugate probe results in a specific assembling of conjugates and can be monitored by light scattering and extinction spectra. To this end, we have developed a special attachment to the Specord M-40 spectrophotometer and a corresponding measurement procedure called by us differential light scattering spectroscopy. This method is suitable for the nearly simultaneous recording of extinction and static light scattering spectra of a simple that possesses overlapping spectral bands of absorption and light scattering.© (2002) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Databáze: OpenAIRE