MP16-11 PREVALENCE OF PROSTATITIS IN MEN TREATED WITH PROSTATECTOMY (OPEN PROSTATECTOMY, TRANSURETHRAL RESECTION OF PROSTATE) FOR BENIGN PROSTATIC HYPERPLASIA INDUCED BLADDER OUTLET OBSTRUCTION

Autor:	Amr Lotfi, Hani El Fayoumy, Hosni Salem, Hosam Shaker, Hesham Fathi, Elia Anis
Rok vydání:	2014
Předmět:	education.field_of_study medicine.medical_specialty business.industry Prostatectomy Urology medicine.medical_treatment Population Prostatitis Hyperplasia medicine.disease Epithelium medicine.anatomical_structure Prostate Medicine Bone marrow business education Open Prostatectomy
Zdroj:	Journal of Urology. 191
ISSN:	1527-3792 0022-5347
Popis:	INTRODUCTION AND OBJECTIVES: Recent studies have reported that bone marrow derived cells (BMDCs), which are recruited to sites of tissue injury and inflammation, can differentiate into epithelial cells, such as liver, lung, gastrointestinal tract, and skin. We investigate the role of BMDCs to contribute to regeneration of injured prostate epithelium and stroma. METHODS: Using chimera rats that had received allogenic bone marrow grafts from green fluorescent protein (GFP) transgenic rats after lethally whole body irradiation, we are able to investigate the contributions of BMDCs to regeneration of prostate epithelium that had injured by transurethral lipopolysaccharide injection. RESULTS: Prostate tissues were harvested at 2, 8, 12, and 32 weeks after transurethral lipopolysaccharide injection, and immunofluorescence staining suggested that BMDCs in the prostate epithelium and storoma co-expressed GFP and pan-cytokeratin, a luminal epithelial marker, but not CK14, a basal epithelial marker. From dissociated prostatic tissue 2 weeks after injury, we collected GFP+/ CD45cell population, which is bone marrow derived non-hematopoietic cells, by fluorescence-activated cell sorter analysis. The number of cells in this population significantly increased from 0.020% to 0.648% compared with normal prostate tissue. Furthermore, by immunofluorescent analysis and RT-PCR, we found that some of these cells (GFP+/CD45-) expressed cytokeratin, which suggest that these cells have some feature of epithelial cell. CONCLUSIONS: Taken together, these results show that BMDCs might be able to differentiate into prostate epithelium and storoma after prostatic injury.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2bc4eab1188eb57af19032c67e6133db https://doi.org/10.1016/j.juro.2014.02.584 Zobrazit plný text záznamu