A novel quantitative volumetric spreading index definition and assessment of astrocyte spreading in vitro
| Autor: | Volkan Mujdat Tiryaki, Ijaz Ahmed, Virginia M. Ayres, David I. Shreiber |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Histology Glial fibrillary acidic protein business.industry Cell Biology Biology computer.software_genre Cell morphology In vitro Pathology and Forensic Medicine 03 medical and health sciences Immunolabeling 030104 developmental biology Optics medicine.anatomical_structure Planar Voxel Biophysics biology.protein medicine business computer Cytometry Astrocyte |
| Zdroj: | Cytometry Part A. 91:794-799 |
| ISSN: | 1552-4922 |
| DOI: | 10.1002/cyto.a.23183 |
| Popis: | A novel quantitative volumetric spreading index (VSI) is defined that depends on the total distance between object voxels and the contact surface plane in three-dimensional (3D) space. The VSI, which ranges from 0 to 1, is rotationally invariant around the z-axis. VSI can be used to quantify the degree of individual cell spreading, which is important for analysis of cell interactions with their environment. The VSIs of astrocytes cultured on a nanofibrillar surface and three different comparative planar surfaces have been calculated from confocal laser scanning microscope z-series images, and the effects of both culture surface and immunoreactivity on the degree of cell spreading were investigated. VSI calculations indicated a statistical correlation between increased reactivity, based on immunolabeling for glial fibrillary acidic protein, and decreased cell spreading. Further results provided a quantitative measure for the increased spreading of quiescent-like and reactive-like astrocytes on planar substrates functionalized with poly-l-lysine. © 2017 International Society for Advancement of Cytometry. |
| Databáze: | OpenAIRE |
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