Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring
| Autor: | Pavel Zemánek, Zbynek Prokop, Martin Kizovsky, Zdenek Pilat, Jaroslav Sobota, Ota Samek, Tereza Klementova, Jan Jezek, Stanislav Kratky, Tomas Buryska, Jiri Damborsky |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
010401 analytical chemistry Microfluidics Substrate (chemistry) Nanotechnology 02 engineering and technology Surface-enhanced Raman spectroscopy 021001 nanoscience & nanotechnology 01 natural sciences Optofluidics 0104 chemical sciences 3. Good health symbols.namesake 13. Climate action Covalent bond symbols 0210 nano-technology Raman spectroscopy Plasmon Haloalkane dehalogenase |
| Zdroj: | 2019 PhotonIcs & Electromagnetics Research Symposium - Spring (PIERS-Spring). |
| Popis: | Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in millimolar and even submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes. |
| Databáze: | OpenAIRE |
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