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Aims: To determine the chemical composition of essential oil of Ageratum conyzoides with antifungal activity on the strain of Lasiodiplodia theobromae in the Kisangani region. Location and Duration of Studies: Faculty of Sciences of University of Kisangani (Democratic Republic of Congo) and Faculty of Biosciences Engineering of University of Ghent (Belgium), between May to November 2019. Methodology: The essential oil from the leaves of A. conyzoides was extracted by hydrodistillation. Potato dextrose agar (PDA) was used as a culture medium. In vitro evaluation of its antifungal activity was performed on PDA medium and expressed as percentage of inhibition (PI). Results: The extracted essential oil (with a yield of 0.63%) showed a PI of 91.63% on the strain of L. theobromae after two days of incubation. It consists of at least 23 compounds, of which seven are in the majority (abundances greater than 1.5% and represent 92.05%), namely Precocene I (38.33%), Beta-caryophyllene (26.51%), Beta-sesquiphellandrene (8.63%), Beta-cubebene (7.91%), Alpha-muurolene (4.95%), 1.5.9.9-Tetramethyl-1.4.7-cycloundecatriene (3.04%), Cis-beta-farnesene (2.99%). The hydrocarbon sesquiterpenes are the most frequent compounds (58.95%). Discussion: Compared to the A. conyzoides saponins the PI obtained from the essential oil is higher. The essential oil chemotype obtained from the leaves of A. conyzoides is of the Precocene I type, similar to the oils from the stems and flowers of the same plant in West Africa. Conclusion: The essential oil of A. conyzoides has a very high inhibitory power on L. theobromae. It mainly contains the Precocene and the hydrocarbon sesquiterpenes. Assessment of the antifungal activity of each separate molecule should be considered. |
