Immobilization of Antibodies on a Photoactive Self-Assembled Monolayer on Gold
| Autor: | Hans Sigrist, B. Michel, Emmanuel Delamarche, H. Biebuyck, Heiko Wolf, H. Ringsdorf, N. Xanthopoulos, Ch. Gerber, H. J. Mathieu, Gajendran Sundarababu |
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| Rok vydání: | 1996 |
| Předmět: |
chemistry.chemical_classification
Scanning electron microscope Stereochemistry Biomolecule Self-assembled monolayer Surfaces and Interfaces Condensed Matter Physics chemistry.chemical_compound chemistry X-ray photoelectron spectroscopy Ellipsometry Covalent bond Monolayer Electrochemistry Benzophenone General Materials Science Spectroscopy Nuclear chemistry |
| Zdroj: | Langmuir. 12:1997-2006 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/la950836t |
| Popis: | This paper presents a strategy for immobilizing biomolecules on a photoactivable surface. A self-assembled monolayer is prepared by adsorbing an ω-functionalized dialkyl disulfide on gold. Functional groups of this monolayer are converted in two steps into a benzophenone derivative with an overall yield of 50 ± 10%. Several independent techniques (ellipsometry, X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy, radiolabel assay, and autoradiography) characterize the reaction and photoimmobilization of antibodies on this surface. The photoimmobilized antibodies cover the surface as a homogeneous and dense monolayer that could not be disrupted by vigorous washing with the detergent Tween 20. Immunoassays demonstrated specific recognition of the immobilized immunoglobulins as measured by their complexation with alkaline phosphatase-linked antibodies. The method of photoimmobilization used here leads to a homogeneous single layer of IgGs, in which the proteins maximize th... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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