Cytokine-Driven Immortalization of in vitro Activated Human T Lymphocytes

Autor:	Keld Kaltoft
Rok vydání:	1998
Předmět:	Telomerase medicine.medical_treatment T cell Immunology CD28 T lymphocyte Biology Cell biology TCIRG1 Cytokine medicine.anatomical_structure Antigen Genetics medicine Cytotoxic T cell Genetics (clinical)
Zdroj:	Experimental and Clinical Immunogenetics. 15:84-89
ISSN:	1421-9948 0254-9670
Popis:	Like other normal human somatic cells, T lymphocytes are believed to have a finite in vitro life span. However, continuous T lymphocyte cell lines can often be established from chronic inflammatory skin diseases when the culture medium is supplemented with IL-2 and IL-4 but without antigen and accessory cells added. Based on the assumption that these continuous T lymphocyte cell lines were activated by antigen during the chronic inflammation taking place in vivo, I investigated whether peripheral blood T lymphocytes could be induced to cytokine-dependent continuous growth following antigen activation. Upon allostimulation, peripheral blood CD4+ T lymphocytes reproducibly escape from cellular senescence. These IL-2- and IL-4-dependent continuous T cell lines show high telomerase activity. Withdrawal of either IL-2 or IL-4 results in cell growth arrest concomitant with down-regulation of telomerase activity. When cultured continuously, these CD4+ human T lymphocytes gradually lose expression of CD28.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2b9bfc498f95df261a8c20b31fdeb546 https://doi.org/10.1159/000019058 Zobrazit plný text záznamu