Isolation and analysis of the total genomic DNA from the date palm (P. dactylifera L.) and related species
| Autor: | S. Al-Awadhi, M. Saleem, Sami Al-Amad, Y. Al-Shayji, F. Al-Salameen |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities biology food and beverages Bioengineering biology.organism_classification Applied Microbiology and Biotechnology Washingtonia robusta DNA extraction body regions Restriction enzyme genomic DNA Cocos nucifera Botany Phoenix dactylifera Restriction digest Palm Biotechnology |
| Zdroj: | Acta Biotechnologica. 14:163-168 |
| ISSN: | 1521-3846 0138-4988 |
| DOI: | 10.1002/abio.370140209 |
| Popis: | The objective of this study was to adapt a plant DNA preparation procedure for the isolation of biologically active DNA and DNA with a high molecular weight from the date palm and other related palms. Mature leaf tissue extractions of the date palm, Phoenix dactylifera L., the coconut tree, Cocos nucifera, and the Mexican Fan Palm, Washingtonia robusta, were characterized for total genomic DNA yield, purity, integrity, as well as restriction digestion and ligation capabilities. It is demonstrated here that the DNA isolation procedure, modified for use with various palm leaf tissues, met the criteria for simplicity and low costs, and yielded DNA of high molecular weight (∼50 Kbp) and of sufficient purity suitable for molecular studies. |
| Databáze: | OpenAIRE |
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