Purification and Characterization of Feruloyl Esterase from Aspergillus awamori
| Autor: | Naoko Nakazato, Kunio Chibana, Shinkichi Tawata, Masanobu Ishihara, Seizen Toyama |
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| Rok vydání: | 1999 |
| Předmět: |
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Chromatography General Chemical Engineering Size-exclusion chromatography Industrial and Manufacturing Engineering Ferulic acid chemistry.chemical_compound Residue (chemistry) Hydrolysis Isoelectric point Column chromatography chemistry Feruloyl esterase Organic chemistry Aspergillus awamori Food Science Biotechnology |
| Zdroj: | Food Science and Technology Research. 5:251-254 |
| ISSN: | 1881-3984 1344-6606 |
| DOI: | 10.3136/fstr.5.251 |
| Popis: | A. awamori feruloly esterases, termed FAE-a and FAE-b, were purified to homogeneity by ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, DEAE-Toyopearl 650 column chromatography, hydrophobic chromatography on Butyl Toyopearl 650S, and gel filtration on Toyopearl HW55. The molecular weights (Mr) of FAE-a and FAE-b were estimated to be 31 KDa and 29 KDa by SDS-PAGE; these isoelectric points were 3.9 and below 3.4, respectively. Both enzymes were optimally active at 45-50°C and pH 5.0-5.5. FAE-a was stable up to 37°C, and FAE-b was stable up to 50°C. Both enzymes hydrolyzed predominantly the feruloylated carbohydrate ester prepared from pineapple stem residue rather than methyl esters of ferulic acid. |
| Databáze: | OpenAIRE |
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