The Dose–Response Curve to X-Rays by Cytokinesis – Block Micronucleus (CMBN) Assay as Biomarker for Dose Estimation in Response Systems to Radiation Emergency
| Autor: | Andreea Grigorescu, Mihaela Oprea, Irina Anca Popescu, Lacramioara Oprica, Andreea Teodor, Manuela Oprisan, Diana Costin |
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| Rok vydání: | 2019 |
| Předmět: |
business.industry
Varian Eclipse Calibration curve Radiation 030218 nuclear medicine & medical imaging 03 medical and health sciences Dose–response relationship 0302 clinical medicine 030220 oncology & carcinogenesis Dose estimation Medicine Biomarker (medicine) Irradiation Micronucleus Nuclear medicine business |
| Zdroj: | 2019 E-Health and Bioengineering Conference (EHB). |
| DOI: | 10.1109/ehb47216.2019.8969934 |
| Popis: | A dose–response curve to X-Rays by Cytokinesis – Block Micronucleus (CMBN) assay was realised for medical dose estimation by the irradiation of blood samples obtained from six healthy donors. The irradiation was performed at room temperature using two X-ray beams at 6MV voltage, the dose rate of 240 cGy / min and the parameters time, monitor units (MU) were chosen using the Varian Eclipse 11 treatment plan system. Two cell culture were initiated for each dose - from 0 to 4 Gy, the final each dose result being reported at 1000 binucleated lymphocytes. For each irradiation dose were analyzed manually 12 000 binucleated lymphocytes. Results were fit with linear-quadratic dose–effect model. Dose Estimate program for fitting calibration curves was used; variations in the background frequency, age and sex categories were also observed, in accordance with results of the cited literature and recommendations for each laboratory to perform in vitro its own dose-response calibration curve. |
| Databáze: | OpenAIRE |
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