Ginkgetin inhibits proliferation of HeLa cells via activation of p38/NF-κB pathway
| Autor: | Yun Li, Jianping Kong, Jianxia Cheng |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
biology Chemistry Cell growth Kinase 030302 biochemistry & molecular biology General Medicine biology.organism_classification Molecular biology HeLa 03 medical and health sciences Real-time polymerase chain reaction MTT assay Tumor necrosis factor alpha Interleukin 8 Fetal bovine serum |
| Zdroj: | Cellular and Molecular Biology. 65:79-82 |
| ISSN: | 1165-158X 0145-5680 |
| DOI: | 10.14715/cmb/2019.65.4.13 |
| Popis: | Effect of ginkgetin on proliferation of human cervical cancer (HeLa) cells and the underlying mechanism were investigated. Human cervical cancer (HeLa) cells were cultured at 37 °C in 10 % fetal bovine serum (FBS) supplemented RPMI 1640 medium in a humidified incubator containing 5 % CO2. Cell proliferation was determined using MTT assay, while real-time quantitative polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to determine the levels of expression of interleukin 1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin 8 (IL-8). The expressions of p38 mitogen-activated protein kinases (p38 MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF- κB) were determined using Western blotting. Treatment of HeLa cells with ginkgetin significantly and time- and dose-dependently inhibited their proliferation (p < 0.05). The invasion of the cells were also significantly and dose-dependently decreased, when compared with control cells (p < 0.05). The expressions of p-p38 and p-NF-κB were significantly and dose-dependently down-regulated, relative to control group (p < 0.05). However, the expressions of p38 and NF-κB in ginkgetin-treated cells were not significantly different from those of control group (p > 0.05). The results of qRT-PCR and ELISA showed that the levels of expression of TNF-α, IL-1β and IL-8 mRNAs were significantly and dose-dependently reduced in HeLa cells after 48 h of treatment with ginkgetin, when compared with the control group (p < 0.05). The anti-proliferative effect of ginkgetin on HeLa cells is exerted via a mechanism involving the p38/NF-κB pathway. |
| Databáze: | OpenAIRE |
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