SEM of a self-expanding vascular stent in a canine animal model
| Autor: | William L. Breckwoldt, L. L. Hayes, Raymond J. Connolly, Allan D. Callow, S. Laliberté Verdon, P. L. Termin, K. E. Gould, M. Belkin, M. J. Allen |
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| Rok vydání: | 1990 |
| Předmět: | |
| Zdroj: | Proceedings, annual meeting, Electron Microscopy Society of America. 48:836-837 |
| ISSN: | 2690-1315 0424-8201 |
| DOI: | 10.1017/s0424820100161746 |
| Popis: | Since the first reported use of a coilspring endovascular prosthesis to improve the outcome of recanalization and ballon angioplasty in peripheral atherosclerotic vessels, a nonthrombogenic, autologous lining has been considered critical for longterm patency. We have investigated the morphology of canine illiac arteries by scanning electron microscopy (SEM) at 3, 7, 21 and 28 days after placement of self-expanding stainless steel stents (Wallstent®).Purpose-bred mongrel dogs, purchased from USDA licensed vendors were housed in the AAALAC approved animal facility at New England Medical Center. Stents (n=18) were placed by percutaneous cannulation into the illiac arteries under fluoroscopic control. The area of each artery containing the stent was excised and perfusion fixed with 3% 0.1M cacodylate buffered glutaraldehyde. Samples were rinsed in 0.1M cacodylate buffer and post-fixed for 60 min. in 0.1M cacodylate buffered 1% Osmium tetroxide. Samples were then rinsed in double-distilled water and fixed for 60 min. in 1% aqueous tannic acid. Samples were dehydrated through graded alcohols, placed in hexamethyldisilazane (HMDS) for 10 minutes and subsequently air dried overnight. Dried specimens were mounted on aluminum stubs with silver paste and examined at 10-20 kv on an Amray 1000B scanning electron microscope. |
| Databáze: | OpenAIRE |
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