IFN-γ Regulates Murine Interferon-Inducible T Cell Alpha Chemokine (I-TAC) Expression in Dendritic Cell Lines and during Experimental Autoimmune Encephalomyelitis (EAE)
| Autor: | N. H. R. Hamilton, J. L. Banyer, A. J. Hapel, S. Mahalingam, A. J. Ramsay, I. A. Ramshaw, S. A. Thomson |
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| Rok vydání: | 2002 |
| Předmět: |
biology
medicine.drug_class business.industry T cell Immunology HLA-DQ2 nutritional and metabolic diseases General Medicine medicine.disease Monoclonal antibody digestive system digestive system diseases Coeliac disease medicine.anatomical_structure Antigen Intestinal mucosa medicine biology.protein Antibody business Gliadin |
| Zdroj: | Scandinavian Journal of Immunology. 55:171-177 |
| ISSN: | 0300-9475 |
| Popis: | The authors have analysed gliadin specific, CD4+ T cells isolated from small intestinal biopsies of 23 adult coeliac disease patients (20 on a gluten-free diet and three untreated) and nine control patients. The biopsies were stimulated ex vivo with a peptic/tryptic digest of gliadin for 24 h, and activated T cells were positively selected with paramagnetic beads coated with an antibody against the interleukin-2 receptor. The T cells were expanded and tested for gliadin reactivity and HLA restriction. Gliadin specific, polyclonal T cell lines were recovered from biopsies of all 23 patients. Inhibition studies of T cell lines from 21 patients with anti-HLA monoclonal antibodies indicated predominant presentation of the gliadin antigen by HLA-DQ2 in T cell lines from 11 patients (lines from seven patients with complete MoAb inhibition, the remaining with incomplete inhibition) and incomplete inhibition by HLA-DR3 in lines from three patients. Nine gliadin specific T cell clones from six patients were established; all of these were HLA-DQ2 restricted. Gliadin specific T cells were not found in biopsies from the non-coeliac controls. Our findings demonstrate that gliadin reactive T cells are commonly found in the intestinal mucosa of CD patients and they support the notion that the majority of T cells recognize gliadin peptide(s) when presented by the disease associated DQ2 molecules. |
| Databáze: | OpenAIRE |
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