Production of 2,3‐butanediol by fermentation of enzymatic hydrolysed bagasse from agave mezcal‐waste using the native Klebsiella oxytoca UM2‐17 strain
| Autor: | Lizbeth Pasaye-Anaya, Alejandra Vargas-Tah, Jesús Campos-García, Agustín Jaime Castro-Montoya, Christian Martínez‐Cámara |
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| Rok vydání: | 2019 |
| Předmět: |
biology
Renewable Energy Sustainability and the Environment Chemistry General Chemical Engineering Organic Chemistry Klebsiella oxytoca Xylose biology.organism_classification Pollution Inorganic Chemistry chemistry.chemical_compound Fuel Technology Butanediol Biofuel 2 3-Butanediol Fermentation Food science Sugar Bagasse Waste Management and Disposal Biotechnology |
| Zdroj: | Journal of Chemical Technology & Biotechnology. 94:3915-3923 |
| ISSN: | 1097-4660 0268-2575 |
| Popis: | BACKGROUND: 2,3‐butanediol is a chemical component with wide industrial applications, such as biofuels and chemical precursors. Reducing 2,3‐butanediol production costs is a high priority and recent focus has been on its biological synthesis from raw renewable materials rather than through conventional chemical procedures. In this study, a 2,3‐butanediol native bacterial producer was selected for the fermentation of Agave cupreata bagasse waste by enzymatic hydrolysation to obtain 2,3‐butanediol. RESULTS: The native Klebsiella oxytoca UM2‐17 strain was selected to efficiently produce 2,3‐butanediol on synthetic mineral media. Using glucose (100 g L⁻¹) and glucose: xylose (50 g L⁻¹:50 g L⁻¹) as sugar sources obtained titres of 29.36 g L⁻¹ and 25.9 g L⁻¹ of 2,3‐butanediol, corresponding to yields of 0.29 g g⁻¹ and 0.26 g g⁻¹, respectively. Agave bagasse solid waste after acid pre‐treatment was utilized to obtain enzymatic hydrolysates, obtaining liquors with glucose/xylose/arabinose at 25.8 g L⁻¹ with efficiency of ∼52.5% sugar conversion from residual solid pre‐hydrolysed bagasse. The production of 2,3‐butanediol through batch fermentation of the enzymatic‐hydrolysate by the UM2‐17 strain showed total depletion of glucose and xylose, achieving a maximal production of 10.3 g L⁻¹ of 2,3‐butanediol plus 0.28 g L⁻¹ ethanol, corresponding to 2,3‐butanediol yield of 0.40 g g⁻¹. CONCLUSION: The results indicate that A. cupreata bagasse waste from mezcal beverage elaboration is a sustainable bioresource for production of 2,3‐butanediol by fermentation of enzymatic bagasse‐hydrolysate using the K. oxytoca UM2‐17 strain. © 2019 Society of Chemical Industry |
| Databáze: | OpenAIRE |
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