| Autor: |
DeLoid, Glen, Yanli Wang, Kapronezai, Klara, Lorente, Laura, Roujie Zhang, Pyrgiotakis, Georgios, Nagarjun Konduru, Ericsson, Maria, White, Jason, Torre-Roche, Roberto De La, Xiao, Hang, McClements, David, Demokritou, Philip |
| Předmět: |
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| Popis: |
Module 1: Assessment of iENM- food interactions (food matrix effect) using food models (Preparation of ENM dispersions in water, Synthesis of food model emulsion, Preparation of Fe2O3 ENM nano-enabled food model); Module 2: Development and assessment of a three stage GIT simulator including mouth, stomach and small intestinal phases for the digestion of nano-enabled food models (Simulated digestion protocol); Module 3: Development and morphological characterization of an in vitro intestinal epithelial model suitable for iENM biokinetics and toxicity studies (Triculture model cell culture method, Measurement of transepithelial electrical resistance (TEER), Triculture immunostaining and imaging for morphological characterization, Triculture cell model TEM characterization; Cellular biokinetics and toxicity experiments (Biokinetics protocol, ICP-MS analysis of biokinetics samples, Cellular Toxicity studies); Colloidal characterization of ENM dispersions, food model and digestae throughout the GIT (Size distribution characterization, Morphological characterization, Dissolution studies for iENM case study); Pristine ENM synthesis and characterization (Synthesis of Fe2O3 ENMs, Pristine ENM characterization) Figure S1: Schematic of biokinetics and toxicity experimental system; Figure S2: Schematic of flame spray pyrolysis setup; Figure S3: TEM image pristine Fe2O3 ENM; Figure S4: Emulsion and digestae morphology by fluorescence microscopy; Figure S5: Cytotoxicity of triculture model; Figure S6: Dissolution of Fe2O3 along GIT; Table S1: Chemicals for simulated digestion; Table S2: Simulated digestion stock and working solutions. (DOCX 5948Â kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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