Characterization of fluidic microassembly for immobilization and positioning of Drosophila embryos in 2-D arrays
| Autor: | Ralph W. Bernstein, Matthew P. Scott, Olav Solgaard, Xiaojing Zhang, Stefan Zappe, Matt Fish |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
animal structures
Yield (engineering) Materials science Metals and Alloys Embryo Condensed Matter Physics Surfaces Coatings and Films Electronic Optical and Magnetic Materials embryonic structures Adhesion force Fluidics Electrical and Electronic Engineering Instrumentation Rotational alignment Biomedical engineering |
| Zdroj: | Sensors and Actuators A: Physical. 114:191-196 |
| ISSN: | 0924-4247 |
| DOI: | 10.1016/j.sna.2003.11.021 |
| Popis: | A technique for the positioning and immobilization of Drosophila embryos in 2-D arrays for use in high throughput microinjection experiments has been characterized. The method is based on fluidic microassembly, and immobilization yield, the number of misplaced embryos, alignment properties, and adhesion force of the embryos have been measured for samples with four different pad geometries. For samples with 250 μm ×400 μm sized rectangular pads, an immobilization yield of 85% was achieved. The adhesion force of the embryos was estimated at 36±22 μN. A substantial amount of clustering was, however, observed. By reducing the pad size to 200 μm ×200 μm and changing the pad pitch and shape, the number of misplaced embryos was reduced to less than 5%. The adhesion force of embryos immobilized at the smaller pads was estimated at 14±5.5 μN, resulting in lower immobilization yield for these samples. The self-assembly process facilitates rotational alignment to some degree, and an average of 40% of the immobilized embryos align within ±9° of the symmetry axis of the immobilization sites. |
| Databáze: | OpenAIRE |
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