Analysis of the toxicity of purothionins and hordothionins for plant pathogenic bacteria
| Autor: | Ph.M. de Vries, D. E. A. Florack, J.W.L. van Vuurde, Willem J. Stiekema, B. Visser |
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| Rok vydání: | 1993 |
| Předmět: |
Bacterial disease
biology fungi food and beverages Xanthomonas campestris pv. Vesicatoria Pathogenic bacteria Plant Science Horticulture biology.organism_classification medicine.disease_cause Microbiology Thionin chemistry.chemical_compound chemistry Plant protein medicine Agronomy and Crop Science Clavibacter michiganensis Bacteria Antibacterial agent |
| Zdroj: | Netherlands Journal of Plant Pathology. 99:259-268 |
| ISSN: | 1573-8469 0028-2944 |
| DOI: | 10.1007/bf01974307 |
| Popis: | Purothionins (PTHs) and hordothionins (HTHs) were purified by cation-exchange chromatography from petroleum-ether extracts of wheat and barley flour respectively. The HTHs could be separated into two fractions, HTH-1 and HTH-2. Radial diffusion assays and micro-plate broth dilution assays with a number of plant pathogenic bacteria showed that these proteins were toxic forClavibacter michiganensis subsp.michiganensis, the causal agent of bacterial canker on tomato,C. m. subsp.sepedonicus, the causal agent of ring rot on potato, andXanthomonas campestris pv.vesicatoria, the causal agent of a spot disease on tomato and pepper. Only minor differences in toxicity between PTHs and HTHs, and between HTH-1 and HTH-2, were detected. Minor differences in toxicity of these thionins were also detected for different strains of these bacteria. The use of these plant proteins for engineering bacterial disease resistance into solanaceous crops will be discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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