Synthesis of triacylglycerol containing conjugated linoleic acid by esterification using two blended lipases
| Autor: | Satoshi Negishi, Yuri Arai, Yoshie Yamauchi-Sato, Tadashiro Hirose |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
chemistry.chemical_classification
Diacylglycerol lipase Chromatography biology General Chemical Engineering Linoleic acid Conjugated linoleic acid Organic Chemistry Triacylglycerol lipase food and beverages Rhizomucor miehei biology.organism_classification chemistry.chemical_compound chemistry Penicillium biology.protein Organic chemistry lipids (amino acids peptides and proteins) Lipase Polyunsaturated fatty acid |
| Zdroj: | Journal of the American Oil Chemists' Society. 83:35-38 |
| ISSN: | 0003-021X |
| Popis: | We have developed an efficient esterification for the synthesis of triacylglycerol (TAG) containing conjugated linoleic acids (CLA) using a blend of two powdered lipases. Two pairs of blended lipases promoted the esterification. Rhizomucor miehei lipase, plus Alcaligenes sp. lipase and Penicillium cammembertii MAG and DAG lipase plus Alcaligenes sp. lipase were used. At the optmal ratio of two lipases, the content of TAG containing CLA (TAG-CLA) in all glycerols reached 82–83% after 47 h using 1 wt% of lipases. With R. miehei lipase plus Alcaligenes sp. lipase, the reaction time to obtain ca. 60% of TAG-CLA was one-third of that needed with R. miehei lipase alone. The optimal ratio of two lipases differed between these two pairs. The optimal ratio was 70–80 wt% of R. miehei lipase in the last stage of the reaction, whereas it was over a wide range of 10–90 wt% for P. camembertii lipase. In the blend of R. miehei lipase plus Alcaligenes sp. lipase, activity remained very high after 10 cycles of esterification (every 47 h) and could be used in the industrial production of TAG-CLA. |
| Databáze: | OpenAIRE |
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