Effects of 1,2-dihydro-4-hydroxy-6-methoxy-N-methyl quinoline in relation to circadian rhythms of NAD+ kinase and NADP+ autoreduction in Neurospora crassa and bioluminescence in Gonyaulax polyedra
| Autor: | H.-P. Kubis, Ruediger Hardeland, Ivonne Balzer |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
0106 biological sciences
Pharmacology 0303 health sciences biology Immunology Conidiation biology.organism_classification 01 natural sciences Neurospora crassa 03 medical and health sciences Biochemistry Darkness Gonyaulax Light emission Circadian rhythm NAD+ kinase Kinase activity 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Comparative Biochemistry and Physiology Part C: Comparative Pharmacology. 102:97-101 |
| ISSN: | 0306-4492 |
| DOI: | 10.1016/0742-8413(92)90050-h |
| Popis: | 1. In Neurospora crassa strain bd a , circadian rhythmicity was investigated in constant darkness. In the growing front of solid agar cultures, conidiation was preceded by a maximum of NAD + kinase activity. The enzyme rhythmicity was suppressed by culturing mycelial discs in liquid medium, but became detectable again when this treatment was followed by 3 hr of air exposure. 2. In air-exposed discs, the circadian maximum of NAD + kinase was enhanced by about 3-fold and advanced by about 3 hr upon administration of 1,2-dihydro-4-hydroxy-6-methoxy- N -methyl-quinoline (DHMMQ), whereas melatonin tended to reduce this rhythm. 3. DHMMQ stimulated the autoreduction of NADP + , the maximum of which followed that of NAD + kinase. 4. Bioluminescence was measured in the dinoflagellate Gonyaulax polyedra , which was transferred from a light/dark cycle of 12:12 hr to constant darkness. 5. DHMMQ stimulated light emission up to more than 13-fold. When given at the low concentration of 10 −6 M, the sensitivity towards DHMMQ varied greatly within the circadian cycle; the maximum of responsiveness occurred 3 hr after the onset of darkness, whereas no effects were seen in the late part of subjective night and during subjective day. |
| Databáze: | OpenAIRE |
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