| Autor: |
Oliveira, BL, Guo, Z, Boutureira, O, Guerreiro, A, Jiménez-Osés, G, Bernardes, GJL |
| Jazyk: |
angličtina |
| Předmět: |
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| Popis: |
The unstrained S-allyl cysteine amino acid was site-specifically installed on apoptosis protein biomarkers and was further used as a chemical handle and ligation partner for 1,2,4,5-tetrazines by means of an inverse-electron-demand Diels-Alder reaction. We demonstrate the utility of this minimal handle for the efficient labeling of apoptotic cells using a fluorogenic tetrazine dye in a pre-targeting approach. The small size, easy chemical installation, and selective reactivity of the S-allyl handle towards tetrazines should be readily extendable to other proteins and biomolecules, which could facilitate their labeling within live cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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