| Popis: |
Replicative double-strand break (DSB) repair has been predominantly studied in single-celled organisms such as bacteria and fungi who lack the germline of metazoans. In contrast, most studies concerning DNA repair in mammals focused on somatic cells while only few authors engaged in embryonic stem cells. Drosophila represents a field of in vivo biochemistry systems linking DNA repair research with the power of classical genetics and modern developmental, molecular and cellular biology. In addition to its biomedical relevance, the Drosophila system helped—until recently—to form evolutionary theory over the past hundred years. There is reasonable evidence that the DSB repair mechanism termed Synthesis-Dependent Strand Annealing (SDSA), represents a candidate primordial repair apparatus opening a better understanding of genome evolution for all organisms. On the basis of in vivo gene knockout targeting experiments exemplified here, recombination tract data reflect the relatedness of the DSB repair mechanics of SDSA, Break Induced Replication (BIR), Single Strand Annealing (SSA), and the involvement of Holliday junctions (HJs) and non-DSB repair pathways. |
