Discrimination of Staphylococcus aureus Strains from Coagulase-Negative Staphylococci and Other Pathogens by Fourier Transform Infrared Spectroscopy
| Autor: | Alexander N. Shchegolikhin, A. B. Shevelev, Ilya N. Kurochkin, Vostrikova Ty, Anastasiia Y. Suntsova, Marina A. Guseva, Rustam R. Guliev, Popov Da |
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| Rok vydání: | 2020 |
| Předmět: |
biology
Chemistry 010401 analytical chemistry Pathogenic bacteria 010402 general chemistry Linear discriminant analysis medicine.disease_cause biology.organism_classification 01 natural sciences 0104 chemical sciences Analytical Chemistry Microbiology Staphylococcus aureus Principal component analysis medicine Typing Coagulase Pathogen Bacteria |
| Zdroj: | Analytical Chemistry. 92:4943-4948 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | Staphylococcus aureus is an extremely infectious and malignant pathogen among many bacteria species. The aim of this work is to provide a robust classification model that would be able to identify S. aureus independent of the culture growth stage and the variations in bacteria concentration in suspension and also one that would be able to identify the pathogen among both taxonomically close species of the same genus and taxonomically distant species of different genera, using Fourier transform infrared spectroscopy (FTIR). In total, the spectra of 141 isolates of 17 bacteria have been used. Based on a combination of principal component analysis (PCA) and linear discriminant analysis (LDA), an identification model providing 100% sensitivity and 98% specificity was built. Inherent reliability and flexibility of the model have been shown. The proposed method of analysis allows us to get closer to the diagnostic requirements in the field of clinical microbiology, and it can be utilized for typing of other pathogenic bacteria species. |
| Databáze: | OpenAIRE |
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