Neonatal Low- and High-Dose Exposure to Estradiol Benzoate in the Male Rat: I. Effects on the Prostate Gland1
Autor: | Ralph L. Cooper, Christian B. Schwartz, Oliver Putz, Gail S. Prins, Steve Kim, Gerald A. LeBlanc |
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Rok vydání: | 2001 |
Předmět: |
medicine.medical_specialty
medicine.drug_class Preputial gland Cell Biology General Medicine Biology medicine.disease chemistry.chemical_compound medicine.anatomical_structure Endocrinology Reproductive Medicine Endocrine disruptor chemistry Estrogen Prostate Dysplasia Internal medicine Toxicity medicine Estradiol benzoate Testosterone |
Zdroj: | Biology of Reproduction. 65:1496-1505 |
ISSN: | 1529-7268 0006-3363 |
DOI: | 10.1095/biolreprod65.5.1496 |
Popis: | Brief exposure of rats to high doses of natural estrogens early in life results in permanent alterations of the prostate gland, which include differentiation defects, altered gene expression, and dysplasia with aging. Whether low-dose treatments can cause similar effects in the developing prostate remains controversial. The current project was designed to determine the dose-response relationship of the prostate gland to estradiol exposure during the developmentally critical neonatal period in the rat. Male Sprague-Dawley (SD) rats were treated on Days 1, 3, and 5 of life by s.c. injections of a 7-log range of doses (0.015 μg/kg to 15.0 mg/kg) of β-estradiol-3-benzoate (EB) in 25 μl of peanut oil (Arachis) as vehicle. In a separate block, neonatal Fisher 344 (F344) rats received 0.15, 15.0, or 1500.0 μg EB/kg. Rats were killed on Postnatal Day (PND) 35 or 90, and the prostates were microdissected, weighed, and frozen for immunohistochemistry. Preputial separation and hepatic testosterone hydroxlas... |
Databáze: | OpenAIRE |
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