Neonatal Low- and High-Dose Exposure to Estradiol Benzoate in the Male Rat: I. Effects on the Prostate Gland1

Autor: Ralph L. Cooper, Christian B. Schwartz, Oliver Putz, Gail S. Prins, Steve Kim, Gerald A. LeBlanc
Rok vydání: 2001
Předmět:
Zdroj: Biology of Reproduction. 65:1496-1505
ISSN: 1529-7268
0006-3363
DOI: 10.1095/biolreprod65.5.1496
Popis: Brief exposure of rats to high doses of natural estrogens early in life results in permanent alterations of the prostate gland, which include differentiation defects, altered gene expression, and dysplasia with aging. Whether low-dose treatments can cause similar effects in the developing prostate remains controversial. The current project was designed to determine the dose-response relationship of the prostate gland to estradiol exposure during the developmentally critical neonatal period in the rat. Male Sprague-Dawley (SD) rats were treated on Days 1, 3, and 5 of life by s.c. injections of a 7-log range of doses (0.015 μg/kg to 15.0 mg/kg) of β-estradiol-3-benzoate (EB) in 25 μl of peanut oil (Arachis) as vehicle. In a separate block, neonatal Fisher 344 (F344) rats received 0.15, 15.0, or 1500.0 μg EB/kg. Rats were killed on Postnatal Day (PND) 35 or 90, and the prostates were microdissected, weighed, and frozen for immunohistochemistry. Preputial separation and hepatic testosterone hydroxlas...
Databáze: OpenAIRE