Growth factors deprivation induces a specific increase in PAR2 receptor mRNA expression in primary cerebellar cultures

Autor: Leonid G. Khaspekov, Natalia V. Gulyaeva, Alexander Yakovlev, A. A. Lyzhin, O. N. Davydova
Rok vydání: 2010
Předmět:
Zdroj: Neurochemical Journal. 4:279-283
ISSN: 1819-7132
1819-7124
DOI: 10.1134/s1819712410040069
Popis: We studied changes in the mRNA expression of protease receptors (PAR-1, PAR-2, PAR-3, and PAR-4) in primary neuroglial and glial cultures of rat cerebellum after a one hour deprivation of culture medium. Replacement of culture medium by saline induced an increase in the mRNA level of PAR-2 in cultures of both types, whereas the mRNA level of other receptors did not change. The maximum expression of PAR-2 was observed in 3 h after the deprivation and in 8 h PAR-2 mRNA returned to the basal level. Incubation of neuroglial cultures in the presence of PAR-2 agonist trypsin (10–100 nM) did not affect expression of PAR-2, however, it induced a specific decrease in the mRNA level of PAR-4. Replacement of trypsin by cathepsin B, which has substrate specificity that is similar to trypsin, did not have this effect. Thus, growth factor deprivation and treatment with trypsin had opposite effects on the mRNA expression of “trypsin” receptors, which may be related to functional role of PAR receptors in the brain.
Databáze: OpenAIRE
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