The spot test is not a reliable screening procedure for mucopolysaccharidoses

Autor:	Ron A. Wevers, A.A.J. van Landeghem, J. J. F. Hasselman, H. L. Vader, J.G.N. de Jong
Rok vydání:	1991
Předmět:	Pathology medicine.medical_specialty Chromatography business.industry Mucopolysaccharidosis Biochemistry (medical) Clinical Biochemistry Dimethylmethylene blue Azure A Urine medicine.disease chemistry.chemical_compound chemistry Reference values Recien nacido medicine Congenital disease business
Zdroj:	Clinical Chemistry. 37:572-575
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/37.4.572
Popis:	To check the reliability of the Ames MPS paper spot test, which is based on the Azure A dye, we sent a series of urine samples to three laboratories where the spot test is part of the metabolic screening for mucopolysaccharidoses. In these laboratories false-negative results ranged between 19% and 35% and false-positive results ranged between 12% and 29% of all samples submitted. In contrast, the quantitative dimethylmethylene blue test (Clin Chem 1989;35:1472-7) detected an increased glycosaminoglycan content in all urine samples from mucopolysaccharidosis patients and gave no false-positive results. In the latter procedure, glycosaminoglycan content is expressed per millimole of creatinine, and age-dependent reference values are used. We conclude that the Ames spot test and other spot tests are unreliable as a screening procedure for mucopolysaccharidoses and should not be used to screen for these diseases.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2adc0b50e079c45b30b918bfc81ef5fb https://doi.org/10.1093/clinchem/37.4.572 Zobrazit plný text záznamu