RETRACTED: The application of quantum dots as fluorescent label to glycoarray

Autor:	Gang-Liang Huang, Tian-Cai Liu, Xin-Ya Mei, Man-Xi Liu
Rok vydání:	2005
Předmět:	Detection limit Electrospray biology Biophysics Nanotechnology Cell Biology Photochemistry Mass spectrometry Biochemistry Fluorescence chemistry.chemical_compound chemistry Concanavalin A Quantum dot biology.protein Azide Fluorescein isothiocyanate Molecular Biology
Zdroj:	Analytical Biochemistry. 340:52-56
ISSN:	0003-2697
Popis:	A sensitive, specific, and rapid method for the detection of carbohydrate-protein interactions was demonstrated using quantum dots (QDs) as a fluorescence label coupled with protein. 1,3-Dipolar cycloaddition between azide and alkyne was exploited to attach α-D-glucopyranoside to a C 1 4 hydrocarbon chain that noncovalently binds to the microtiter well surface, and the product formation was detected by both electrospray ionization-mass spectrometry (ESI-MS) and QD- (or fluorescein isothiocyanate (FITC))-conjugated lectin binding. It indicated that the peak intensity of the fluorescence emission was proportional to the initial concanavalin A (Con A) concentration in the range of 2 × 10 - 3 μmol/L to 2 x 10 - 2 n-imol/L with a detection limit at least 100 times lower than that of the FITC-based method.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2acf19ff5b2127eca40e43cbc26c8d71 https://doi.org/10.1016/j.ab.2005.01.013 Zobrazit plný text záznamu Full Text from ScienceDirect