Double-antibody fluorescence immunoassay of tobramycin

Autor:	H T Karnes, J D Winefordner, J C Gudat, C M O'Donnell
Rok vydání:	1981
Předmět:	Chromatography biology Chemistry Biochemistry (medical) Clinical Biochemistry Radioimmunoassay Assay sensitivity Fluorescence immunoassay Fluorescence carbohydrates (lipids) Antigen Double antibody biology.protein Tobramycin medicine Antibody medicine.drug
Zdroj:	Clinical Chemistry. 27:249-252
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/27.2.249
Popis:	This heterogeneous assay for tobramycin involves fluorescein-labeled tobramycin, which competes with native unlabeled tobramycin for anti-tobramycin binding sites. Bound and free labeled antigen are separated by precipitation with a second antibody. Fluorescence intensity of the resuspended precipitate is inversely proportional to native tobramycin concentration. Background interference was consistently about 10% of the total fluorescence precipitated. Assay sensitivity was sufficient to detect nanogram quantities of tobramycin per assay tube. Correlation coefficients (r) were 0.96 and 0.98 for comparisons of this assay with a microbiological assay and a radioimmunoassay, respectively. Mean analytical recovery was 101% and the CV was less than 10% throughout the therapeutic range.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2ac97b857ad04ea456b045294c0ab309 https://doi.org/10.1093/clinchem/27.2.249 Zobrazit plný text záznamu