| Popis: |
A new method measures the spectral absorption of small volume samples having peak optical densities ( D p ) too high (up to 50 and higher) to be measured by standard direct or indirect methods. This method specifically corrects for the effect on the microspectrophotometer (MSP) of wavelength-independent light which bypasses the sample. The method involves: (1) measuring by MSP the long wavelength cutoff of the dense samples and the absorption spectrum of thinned samples of the same pigment, (2) calculating D p from these. The method's application is illustrated for the colored oil droplets in the retinal cones of the turtle, Emydoidea blandingii . |
