Are Compounds Membrane-Associated or Present in the Cytosol? A Study Using Polyphenols in a Colon Carcinoma Cell Line Model
| Autor: | Holger Barth, Isabel Anna Maria Groh, Tamara Bakuradze, Eva Kaiser, Elke Richling, Nadine Simson, Hannah Deusser |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pharmacology 030102 biochemistry & molecular biology Chemistry Phloretin food and beverages Biological activity Biochemistry In vitro Cell membrane 03 medical and health sciences Cytosol chemistry.chemical_compound 030104 developmental biology Digitonin medicine.anatomical_structure Polyphenol Drug Discovery Genetics medicine Quercetin |
| Zdroj: | Current Pharmacology Reports. 6:451-456 |
| ISSN: | 2198-641X |
| Popis: | Purpose We investigated the cytosolic and membrane-associated contents of polyphenols after 4 hours of incubation (50 μM of each polyphenol) in the colon carcinoma cell line T84 using a novel, rapid, and convenient method based on permeabilization of the cell membrane using digitonin. The colon carcinoma cell line was used to investigate the intestinal uptake of polyphenols present in apple products. Recent Findings The results showed that hydroxycinnamic acids (caffeic and 5-caffeoylquinic acid) were only detected in the cytosolic fractions. In contrast, 0.3 to 8.2% of the initial concentrations (50 μM) of the flavonoids phloretin, quercetin, phloretin 2′-O-glucoside, and quercetin 3-O-rhamnoside were found in the membrane-associated fractions. In the cytosolic fractions, 0.2–2.9% of these compounds were detected, corresponding to 25 to 40% of the total cell-associated (cytosolic plus membrane-associated fractions) polyphenol content. Summary Our results showed that after uptake, polyphenols were present in the cytosolic fraction of the cells as well as associated with the cell membrane. The presented method provides a useful in vitro tool for determining biologically active compounds in cellular fractions. |
| Databáze: | OpenAIRE |
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