Extraction and purification of flavanone glycosides and kaemferol glycosides from defatted Camellia oleifera seeds by salting-out using hydrophilic isopropanol

Autor: Chieh-Ming J. Chang, Bing-Chung Liau, Ting-Ting Jong, Jung-Hui Chen
Rok vydání: 2009
Předmět:
Zdroj: Separation and Purification Technology. 67:31-37
ISSN: 1383-5866
DOI: 10.1016/j.seppur.2009.02.020
Popis: The purpose of this research was to investigate a salting-out procedure for isolating four flavonoid glycosides from defatted Camellia oleifera seeds. The procedure included extraction with 80% methanol, methanol removal and addition of an equal amount of hydrophilic isopropanol and salt to separate the isopropanol fraction from the water layer. Using successive column chromatography, kaemferol-3-O-[2-O-β- d -glucopyranosyl-6-O-α- l -rhamnopyranosyl]-β- d -glucopyranoside (compound 1), kaemferol-3-O-[2-O-β- d -xylopyranosyl-6-O-α- l -rhamnopyranosyl]-β- d -glucopyranoside (compound 2), naringenin-7-O-[β- d -xylopyranosyl(1 → 6)][β- d -glucopyranosyl(1 → 3)-α- l -rhamnopyranosyl(1 → 2)]-β- d -glucopyranoside (compound 3) and naringenin-7-O-β- d -xylopyranosyl(1 → 6)-β- d -glucopyranoside (compound 4) were obtained. The structure of compound 3, a new flavanone glycoside, was analyzed using UV, FT-IR, 1H NMR, 13C NMR and HR-FAB-MS. Quantification using high-performance liquid chromatography (HPLC) demonstrated that defatted C. oleifera seed cake contains 7.92, 17.7, 2.23 and 1.06 mg/g of the four compounds. The extraction efficiencies of the four compounds were increased by 23.17%, 7.59%, 48.67% and 47.22% from those obtained with n-butanol partition extraction. This new extraction technique is simpler, faster and less expensive than the traditional extraction method. Accordingly, this salting-out method can potentially replace the existing one.
Databáze: OpenAIRE
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