Preparation of [11C]-thymidine and [11C]-2′-arabino-2′-fluoro-β-5-methyl-uridine (FMAU) using a hollow fiber membrane bioreactor system
Autor: | Neil G. Hartman, Jeffrey A. Hughes, Michael Jay |
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Rok vydání: | 1995 |
Předmět: |
Chromatography
Immobilized enzyme Organic Chemistry technology industry and agriculture Substrate (chemistry) equipment and supplies complex mixtures Biochemistry Uridine Analytical Chemistry Alcohol oxidase chemistry.chemical_compound Membrane chemistry Hollow fiber membrane Drug Discovery Bioreactor Organic chemistry Radiology Nuclear Medicine and imaging Nucleoside Spectroscopy |
Zdroj: | Journal of Labelled Compounds and Radiopharmaceuticals. 36:1133-1145 |
ISSN: | 0362-4803 |
DOI: | 10.1002/jlcr.2580361204 |
Popis: | A series of hollow fiber membranes containing immobilized enzymes were prepared and used in the synthesis of 11 C-labelled nucleosides. 11 C-Formaldehyde was produced in an alcohol oxidase/catalase bioreactor and circulated through a thymidylate synthase bioreactor with an appropriate substrate to produce the corresponding 11 C-nucleotide. These labelled nucleotides were subsequently dephosphorylated in an alkaline phosphatase bioreactor. The bioreactor approach was amenable to hot-cell conditions and yielded 11 C-products in higher yield and shorter synthesis times than conventional chemical approaches. |
Databáze: | OpenAIRE |
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