Preparation of [11C]-thymidine and [11C]-2′-arabino-2′-fluoro-β-5-methyl-uridine (FMAU) using a hollow fiber membrane bioreactor system

Autor: Neil G. Hartman, Jeffrey A. Hughes, Michael Jay
Rok vydání: 1995
Předmět:
Zdroj: Journal of Labelled Compounds and Radiopharmaceuticals. 36:1133-1145
ISSN: 0362-4803
DOI: 10.1002/jlcr.2580361204
Popis: A series of hollow fiber membranes containing immobilized enzymes were prepared and used in the synthesis of 11 C-labelled nucleosides. 11 C-Formaldehyde was produced in an alcohol oxidase/catalase bioreactor and circulated through a thymidylate synthase bioreactor with an appropriate substrate to produce the corresponding 11 C-nucleotide. These labelled nucleotides were subsequently dephosphorylated in an alkaline phosphatase bioreactor. The bioreactor approach was amenable to hot-cell conditions and yielded 11 C-products in higher yield and shorter synthesis times than conventional chemical approaches.
Databáze: OpenAIRE