Préparation de produits de thérapie cellulaire: apport des systèmes clos

Autor:	Jean-Marie Certoux, E. Rousseau, Pierre Tiberghien, R. Hervé, I. Newton, B. Petracca, Eric Robinet, N. Roubi
Rok vydání:	1999
Předmět:	Genetic enhancement medicine.medical_treatment Biochemistry (medical) Clinical Biochemistry Gene transfer Hematology Hematopoietic stem cell transplantation Biology Pharmacology Cell selection Cell handling Cell therapy Immunology medicine Stem cell Ex vivo
Zdroj:	Transfusion Clinique et Biologique. 6:409-417
ISSN:	1246-7820
DOI:	10.1016/s1246-7820(00)88986-7
Popis:	A phase I clinical trial is being currently performed in our institution, aiming at evaluating the feasibility and toxicity related to the administration of Herpes Simplex-thymidine kinase gene-expressing human primary T lymphocytes following allogeneic hematopoietic stem cell transplantation. The need for safe and standardized preparation conditions for gene-modified cells is crucial. We describe the closed culture system used in the current trial for ex vivo retroviral-mediated gene transfer and transduced cell selection. Cell handling is performed in closed systems using sampling and transfer pack bags, culture bags and a sterile connection device which avoids opening the culture system. This closed system allows safe and reproducible ex vivo preparation of gene-modified primary T-lymphocytes for clinical use.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2a90c162ffeff5262272edcdfe55987d https://doi.org/10.1016/s1246-7820(00)88986-7 Zobrazit plný text záznamu Full Text from ScienceDirect