Indirect Competitive Enzyme-Linked Immunosorbent Assay for Detection of Tylosin in Milk and Water Samples
| Autor: | Yu-Dong Shen, Hong Wang, Jin-Yi Yang, Zhen-Lin Xu, Hongtao Lei, Chan-Yuan Yao, Yuanming Sun, Yuanxin Tian |
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| Rok vydání: | 2018 |
| Předmět: |
Detection limit
chemistry.chemical_classification Chromatography biology 010401 analytical chemistry 02 engineering and technology Raw milk Tylosin 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Enzyme Linear range chemistry Polyclonal antibodies biology.protein Antibody 0210 nano-technology Hapten |
| Zdroj: | Chinese Journal of Analytical Chemistry. 46:1275-1281 |
| ISSN: | 1872-2040 |
| DOI: | 10.1016/s1872-2040(18)61106-5 |
| Popis: | A specific polyclonal antibody was prepared based on a new hapten with stable structure. Based on this, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was established for determination of tylosin residues in food and environmental samples. The experimental conditions were optimized as follows: the coating antigen and antibody were respectively diluted by 6000 times and 2000 times, and the competitive reaction time was 40 min. Under the optimized conditions, the method showed a detection limit of 0.07 ng mL−1, an IC50 of 1.39 ng mL−1 and a linear range of 0.17–11.0 ng mL−1. The recoveries of spiked raw milk and water samples were ranged from 78.4% to 105.6%, with the RSDs of less than 15%. Good correlation between icELISA and HPLC method was obtained for spiked samples (R2 = 0.97). This method was suitable for the determination of tylosin residues in milk and water samples. |
| Databáze: | OpenAIRE |
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