PRIMITIVE ERYTHROPOIESIS IN YOLK SAC EXPLANTS IS INHIBITED BY ANTISENSE ERYTHROPOIETIN-RECEPTOR, c-RAF, AND JAK2 OLIGODEOXYNUCLEOTIDES (ODN). • 940
| Autor: | James Palis, Jane L. Liesveld, Judeth K. McGann, Louise Silver |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Mesoderm
medicine.medical_treatment In situ hybridization Biology Molecular biology Erythropoietin receptor medicine.anatomical_structure Cytokine Erythropoietin hemic and lymphatic diseases embryonic structures Pediatrics Perinatology and Child Health Immunology medicine c-Raf Yolk sac Receptor medicine.drug |
| Zdroj: | Pediatric Research. 39:159-159 |
| ISSN: | 1530-0447 0031-3998 |
| DOI: | 10.1203/00006450-199604001-00962 |
| Popis: | Erythropoietin (EPO) binds its specific receptor (EPO-R) to mediate survival and proliferation of definitive (bone marrow/fetal liver) red cell precursors. In contrast, little is known about cytokine signaling inprimitive (yolk sac) erythroblasts. We detected EPO-R mRNA by in situ hybridization at early stages of murine yolk sac blood island development(E7.5-E8.5). Because the size and location of the mammalian embryo makes experimental manipulation difficult, we developed a serum-free murine yolk sac explant culture system {Blood 86:156, 1995}, and an antisense experimental approach to examine the functional role of EPO/EPO-R during the initial development of primary primitive erythroblasts from extra-embryonic mesoderm cells. The addition of EPO to E7.5 yolk sac explants increased both the accumulation of βH1-globin mRNA and the number of differentiating yolk sac erythroblasts compared to untreated explants. Antisense EPO-R ODN decreased βH1-globin accumulation 95% (p 50% (p |
| Databáze: | OpenAIRE |
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