Integrated microRNA and mRNA sequencing analysis of age-related changes to mouse thymic epithelial cells

Autor: Hong-Ling Jia, Yugu Li, Dong-Guang Guo, Jiang-Hui Zeng, Zhuo-Ya Wang, Ya-Meng Liu, Bishuang Gong, Xiao-Qin Zeng, Kai-Zhao Zhang, Feng Huang
Rok vydání: 2018
Předmět:
Zdroj: IUBMB Life. 70:678-690
ISSN: 1521-6543
DOI: 10.1002/iub.1864
Popis: MicroRNAs (miRNAs) play key roles in the regulation of gene expression during multiple physiological processes, including early development, differentiation, and ageing. However, their involvement in age-related thymic involution is not clear. In this study, we profiled the global transcriptome and miRNAome of thymic epithelial cells in 1- and 3-month-old male and female mice, and predicted the possible transcription factors and target genes of the four most significantly differentially expressed miRNAs (DEMs) (miR-183-5p, miR-199b-5p, miR-205-5p, and miR-200b-3p) by performing bioinformatics analyses. We also evaluated the relationships between the significantly DEMs and mRNAs. We performed quantitative polymerase chain reaction to confirm the changes in the expression of the miRNAs and their predicted target genes. We found that miR-183-5p, miR-199b-5p, miR-205-5p, and miR-200b-3p can be used as a biomarker group for mouse thymus development and involution. In addition, the predicted target genes (Ptpn4, Slc2a9, Pkib, Pecam1, and Prkdc), which were identified by mRNA sequencing analysis, were mainly involved in growth, development, and accelerated senescence. In conclusion, miRNAs and their predicted target genes likely play important roles in thymus development and involution. To the best of our knowledge, this is the first study to systematically analyze the relevance of miRNAs and their targets by mRNA sequencing in mouse thymic epithelial cells. © 2018 IUBMB Life, 70(7):678-690, 2018.
Databáze: OpenAIRE
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