Separate and combined effects of caffeine and dbcAMP on olive baboon (Papio anubis) sperm
| Autor: | Thomas M. D’Hooghe, Daniel C. Chai, Nicholas M. Kiulia, Atunga Nyachieo, Carl Spiessens, Jason M. Mwenda |
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| Rok vydání: | 2010 |
| Předmět: |
endocrine system
In vitro fertilisation General Veterinary biology urogenital system medicine.medical_treatment Semen Sperm Andrology chemistry.chemical_compound medicine.anatomical_structure chemistry Capacitation biology.animal embryonic structures medicine Animal Science and Zoology Zona pellucida Caffeine reproductive and urinary physiology Sperm motility Baboon |
| Zdroj: | Journal of Medical Primatology. 39:137-142 |
| ISSN: | 1600-0684 0047-2565 |
| DOI: | 10.1111/j.1600-0684.2010.00401.x |
| Popis: | Background Improvement of baboon sperm capacitation is necessary for achieving high in vitro fertilization (IVF) rates in baboons. In this study, we evaluated separate and combined effects of caffeine and dbcAMP on baboon sperm capacitation. Methods Sixteen male baboons (n = 16) were electroejaculated. Each sperm sample was divided into two aliquots: one for chemical activation and the other untreated control. Group 1: dbcAMP (n = 6); Group 2: caffeine (n = 6) and Group 3: combination of caffeine and dbcAMP (n = 4). In each aliquot, sperm motility after 30 minutes of incubation was evaluated as well as zona pellucida (ZP) binding ability after overnight incubation with 4–5 ZP from unfertilized human oocytes. Results Sperm motility and ZP binding ability in all chemically activated groups increased significantly as compared to their respective controls (P |
| Databáze: | OpenAIRE |
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