Assessment of Caveolae/Lipid Rafts in Isolated Cells
| Autor: | Glaucia E. Callera, Rhian M. Touyz, Thiago Bruder-Nascimento |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cholesterol Cellular functions Biology Sphingolipid Protein subcellular localization prediction Cell biology Cell membrane 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology medicine.anatomical_structure Biochemistry chemistry Caveolae medicine Phosphorylation Lipid raft |
| Zdroj: | Hypertension ISBN: 9781493966233 |
| Popis: | This chapter outlines protocols to evaluate protein localization, recruitment or phosphorylation levels in cholesterol/sphingolipids-enriched cell membrane domains and recommends experimental designs with pharmacological tolls to evaluate potential cell functions associated with these domains. We emphasize the need for the combination of several approaches towards understanding the protein components and cellular functions attributed to these distinct microdomains. |
| Databáze: | OpenAIRE |
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