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Introduction Hypertensive disorders in pregnancy are serious complications of maternal and/or fetal well being. Hypertension solely (PIH) or preeclampsia (PE), a pregnancy-associated specific disorder characterized by hypertension and excess protein excretion in the maternal urine, are important causes of maternal and fetal morbidity and mortality worldwide. Objectives The etiology of disorder is multi-factorial, including the imbalance between pro- and anti-inflammatory cytokines in impaired immune system. In this study we aimed to analyze the role of IL-10 592 C>A (rs1800872) in healthy pregnant women at term, women with preeclampsia and hypertension, and investigated whether this polymorphism has impact on disease risk and severity. Methods A 100 subjects of whom 36 were preeclamptic patients, 45 healthy pregnant controls and 19 women with PIH were involved in case-control study. Levels of proteinuria for 24 hours were measured and correlated to the IL-10 genotype. The genomic DNA was isolated from peripheral blood leukocytes and genotyping was performed by using TaqMan Universal PCR Mastermix and TaqMan SNP Genotyping Assays for rs1800872on AB7500 Fast Real-Time PCR System. Retrieved results have been correlated to clinico-pathological variables and risk assessment of the disease. For statistical analyses, non-parametric methods were applied. Results There was increased risk for pregnancy induced hypertensive disorders (PE + PIH) in women with aberrant (A) allele in genotype in crude analysis showing the OR = 1.25 (CI95%:0.56–2.76) for subjects carrying genotypes (C/A + A/A). The risk for PE was higher than risk for cases only with hypertension, (OR = 1.31; CI95%:0.54–3.15 vs. OR = 1.16; CI95%:0.39–3.40), respectively. Moreover, there was noted a positive correlation between -592A IL-10 genotype and PE with protein excretion >1 g/24;ħ(OR = 1.35; 95%CI:0.34–5.43 for C/A genotype and OR = 1.84; 95%CI:0.27–12.35 for A/A homozygote). Although insignificant association between IL-10 592C > A and onset of the PE was assessed, a slight trend for higher prevalence of early form ( Conclusion The risk of hypertensive disorders in pregnancy may be elevated among Slovak women with IL-10 592C>A polymorphisms, where the A allele may be associated with severe cases of PE. |
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