Optimization of phenol degradation by Antarctic bacterium Rhodococcus sp
| Autor: | Azham Zulkharnain, Tengku Athirrah Tengku-Mazuki, Kavilasni Subramaniam, Gillian Li Yin Lee, Peter Convey, Siti Aqlima Ahmad, Nur Nadhirah Zakaria, Noor Azmi Shaharuddin, Khalilah Abdul Khalil |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Strain (chemistry) Central composite design biology Chemistry Geology 010501 environmental sciences Bacterial growth Oceanography biology.organism_classification 01 natural sciences Salinity 03 medical and health sciences chemistry.chemical_compound Bioremediation Phenol Response surface methodology Ecology Evolution Behavior and Systematics Bacteria 030304 developmental biology 0105 earth and related environmental sciences Nuclear chemistry |
| Zdroj: | Antarctic Science. 32:486-495 |
| ISSN: | 1365-2079 0954-1020 |
| DOI: | 10.1017/s0954102020000358 |
| Popis: | This study focused on the ability of the Antarctic bacterium Rhodococcus sp. strain AQ5-14 to survive exposure to and to degrade high concentrations of phenol at 0.5 g l-1. After initial evaluation of phenol-degrading performance, the effects of salinity, pH and temperature on the rate of phenol degradation were examined. The optimum conditions for phenol degradation were pH 7 and 0.4 g l-1 NaCl at a temperature of 25°C (83.90%). An analysis using response surface methodology (RSM) and the Plackett-Burman design identified salinity, pH and temperature as three statistically significant factors influencing phenol degradation. The maximum bacterial growth was observed (optical density at 600 nm = 0.455), with medium conditions of pH 6.5, 22.5°C and 0.47 g l-1 NaCl in the central composite design of the RSM experiments enhancing phenol degradation to 99.10%. A central composite design was then used to examine the interactions among these three variables and to determine their optimal levels. There was excellent agreement (R2 = 0.9785) between experimental and predicted values, with less strong but still good agreement (R2 = 0.8376) between the predicted model values and those obtained experimentally under optimized conditions. Rhodococcus sp. strain AQ5-14 has excellent potential for the bioremediation of phenol. |
| Databáze: | OpenAIRE |
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