Identification of a Novel Animal Metabolite of Methomyl Insecticide
| Autor: | Robert W. Reiser, William G. Payne, William T. Zimmerman, David L. Ryan, Arthur J. Fogiel, Robert F. Dietrich, Tanya K. S. Djanegara |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Carbamate
Chromatography medicine.medical_treatment Electrospray ionization Metabolite Methomyl General Chemistry Reversed-phase chromatography Mass spectrometry High-performance liquid chromatography chemistry.chemical_compound chemistry medicine Methylene General Agricultural and Biological Sciences |
| Zdroj: | Journal of Agricultural and Food Chemistry. 45:2309-2313 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf960966n |
| Popis: | In studies conducted to determine the fate of methomyl in goat, chicken, and monkey, an unknown highly polar, acidic metabolite was found in the urine/excreta of all three animals. The metabolite was purified by HPLC and analyzed by microcolumn LC/MS with electrospray ionization and by NMR. A Hypercarb porous graphitic carbon HPLC column was used for purification and LC/MS, since the unknown was unretained on conventional reversed phase HPLC columns. [14C]Methomyl was used as radiotracer, and [13C]methomyl was added at the 50% level to facilitate metabolite identification. The molecular weight of the metabolite was determined by LC/MS-electrospray, using positive and negative ionization, and structurally useful fragment ions were obtained in both modes by in-source collision-induced dissociation (CID). High-resolution accurate mass measurements of the MH+ doublet allowed assignment of the elemental composition. NMR data showed the presence of methyl, methylene, and methine protons with coupling observed b... |
| Databáze: | OpenAIRE |
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