| Popis: |
Dr. Baumgarten : In summarizing this discussion, it is apparent that standardization of fibrinolytic agents should be carried out by measuring the lysis of a fibrin clot. Most investigators agree on this. However, in addition, other types of assays may well be considered as adjuncts. Breakdown of an assay into proteolytic and activator activities can be carried out by employing casein as the substrate of choice. Some investigators prefer heated and unheated fibrin plates for such differential assays. It is a matter of preference of the investigator as to which assay procedure is employed. However, for purposes of standardization, one method should be stipulated as the primary assay. I recommend casein as the substrate of choice, as the results obtained with it are more reproducible than those obtained with the fibrin plate. Apparently casein has been used extensively in many laboratories throughout the world. Thus, while the clot lysis test assays the sum of proteolytic and activator activity, individual assay of the proteolytic and activator activity can be achieved with casein substrate. |
