Potato Leaf Roll Virus 3' Genome Organization: Sequence of the Coat Protein Gene and Identification of a Viral Subgenomic RNA

Autor: O. P. Smith, K. F. Harris
Rok vydání: 1990
Předmět:
Zdroj: Phytopathology. 80:609
ISSN: 0031-949X
DOI: 10.1094/phyto-80-609
Popis: (...) The coat protein gene contains 624 nucleotides and encodes a protein of 208 amino acids with a calculated molecular weight of 23.2 kDa. This gene is located internal to the 3'-end of the genome, contains the complete overlap of an open reading frame (ORF) encoding a 17.4 kDa protein, and is followed in-frame by an ORF encoding a 56.4 kDa protein. Northern blot hybridization experiments have identified a 3'-positioned viral subgenomic RNA produced in PLRV-infected plants. This subgenomic RNA is not encapsidated and, by analogy to other RNA plant viruses, is likely the mRNA for expression of the 3'-portion of the genome (...)
Databáze: OpenAIRE