Determining a Diagnostic Cut-Off on Fluorescence Polarization Assay (FPA) for Bovine Brucellosis in Carchi, Ecuador
| Autor: | García Judith, Salgado Ruth, Ibarra Marcelo, Herrera David, Puga Byron, Mina Jorge, Campos Martín, Peña Julio, Benavides Hernán, Gutiérrez Milena |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Veterinary medicine Receiver operating characteristic biology 040301 veterinary sciences business.industry Brucellosis 04 agricultural and veterinary sciences Brucella 030108 mycology & parasitology medicine.disease biology.organism_classification Serology 0403 veterinary science 03 medical and health sciences Bovine brucellosis Immunology medicine Herd Cutoff Cut-off business |
| Zdroj: | Open Journal of Animal Sciences. :425-432 |
| ISSN: | 2161-7627 2161-7597 |
| DOI: | 10.4236/ojas.2017.74033 |
| Popis: | Serology is the foundation of any brucellosis control and eradication program worldwide, thus it is important to define accuracy diagnostics assays and cut-off of those assays, due to variations from country to country and even among specific areas in the country. The variation of cutoff values depended on: prevalence of disease, vaccination status, animal management, and control and eradication programs. Therefore, a cut-off for the diagnosis of bovine brucellosis through fluorescence polarization assay (FPA) in Carchi—Ecuador was determined. The survey has been carried out in Carchi province of Ecuador, who is considered a province of high prevalence of brucellosis and the vaccination status is unknown due to the lack of registers. Sera samples (n = 200) were obtained from individual cows from randomly selected herds. Blood sera were tested through Fluoresce Polarization Assay (FPA) and competitive enzyme-linked inmunosorbent assay (cELISA) as confirmatory test, and then receiver operating characteristic (ROC) analysis was done. The sensitivity and specificity values of FPA were 88.7% and 92.50% respectively using a cut-off of 89.90 mP. Moreover, the area under the curve showed that 92.2% is the probability accuracy of the test. The advantage of the FPA is that it is a test with good characteristics of sensitivity and specificity as well as a simple and quick test. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|