| Autor: |
A.G. Perez, M.L. Eidinoff, A.S. Gelbard, J.H. Kim |
| Rok vydání: |
1967 |
| Předmět: |
|
| Zdroj: |
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 134:388-394 |
| ISSN: |
0005-2787 |
| DOI: |
10.1016/0005-2787(67)90015-9 |
| Popis: |
1. A mitotically synchronized population of HeLa cells was exposed to 5-bromodeoxyuridine for 3-h intervals spaced over the entire division cycle. The maximum loss in cell viability took place when the exposure to 5-bromodeoxyuridine occurred during the interval when the rate of DNA synthesis was a maximum. 2. Following a 24-h exposure to the analog, DNA synthesis was inhibited in the subsequent replication cycle whereas RNA and protein synthesis was slightly reduced. 3. The activity of DNA polymerase (deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) and uridine kinase (ATP:uridine 5′phosphotransferase) was reduced by a significantly large percentage relative to changes in total cellular protein. This may imply alteration in functional protein consequent to the replacement of DNA thymine by 5-bromouracil. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
